Fig. 5From: Characterization of ACE2 naturally occurring missense variants: impact on subcellular localization and traffickingACE2 variants glycosylation profile. HEK293 cells transiently co-transfected transfected with Flag-tagged ACE2 WT or missense variants and GFP plasmids for 48Â Hrs. Lysates were then digested in presence or absence of Endo H enzymes for 3Â Hrs. Anti-Flag primary antibody was used to stain WT ACE2 and anti-GFP antibody was used to satin GFP that was used as a transfection control. Actin was used as a loading control. Images were manipulated using ImageJBack to article page