Fig. 2

Ectopic expression of Circ_0000915 promoted propranolol resistance of HemSCs. A qRT-PCR confirmed the overexpression efficiency of Circ_0000915 in HemSCs transfected with Circ_0000915 plasmid (Circ-oe) or empty vector (Vector). B CCK-8 assay showed cell viability of HemSCs exposed to different concentration of propranolol for 72 h after transfection with Circ-oe or Vector. C IC₅₀ value of propranolol (72 h treatment) was calculated after HemSCs were transfected with Circ-oe or Vector. D, E Expression of proliferative-related markers (Cyclin D1 and PCNA) in Circ_0000915-overexpressing HemSCs exposed to propranolol (20 μM) for 48 h was measured by qRT-PCR and Western blot. F, G Cell apoptosis of Circ_0000915-overexpressing HemSCs exposed to propranolol (20 μM) for 48 h were determined by caspase-3 activity assay (F) and Annexin-V/PI double staining assay (G). The tumor growth curve (H), the tumor volume (I) and weight (J) at the end point (day 35) derived from Circ_0000915-overexpressing HemSCs. Data are presented as mean ± S.D from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. (Two-way ANOVA for B and H, Student’s t-test for others)