Fig. 3

IL-6 induced Circ_0000915 expression via activation of STAT3. A Expression of Circ_0000915 in HemSCs treated with the indicated concentration of IL-6 for 24 h was detected by qRT-PCR. B STAT3 phosphorylation in HemSCs treated with the indicated concentration of IL-6 for 24 h was examined by Western blot. C qRT-PCR analysis of Circ_0000915 expression in HemSCs cells transfected with STAT3 siRNAs (pool) or negative control siRNAs, followed by treatment with 40 ng/ml IL-6 or PBS for 24 h. D Knockdown efficiency of STAT3 in HemSCs cells transfected with STAT3 siRNAs (pool) or negative control siRNAs was confirmed by Western blot. E HemSCs was co-transfected with either STAT3 siRNAs (pool) or negative control siRNAs plus the Circ_0000915 promoter reporter constructs and Renilla luciferase plasmid. 48 h after transfection, reporter activity was measured and plotted after normalizing with respect to Renilla luciferase activity. F ChIP assays in HemSCs using a STAT3 antibody, followed by qRT-PCR analysis of Circ_0000915 promoter enrichment. Data are presented as mean ± S.D from three independent experiments. *P < 0.05; **P < 0.01; ns = not significant. Student’s t-test