Fig. 4

Circ_0000915 negatively regulated the expression of miR-890 in HemSCs. A Distribution of Circ_0000915 in nucleus and cytoplasm of HemSCs was confirmed by qRT-PCR. B The prediction for miR-890 binding sites on Circ_0000915 and schematic of luciferase reporter vector constructs Circ_0000915 wild-type (Circ_0000915-wt) and the miR-890-binding-site mutated (Circ_0000915-mt) one. C, D The luciferase activities in HemSCs and HEK 293t cells co-transfected with miR-890 or miR-NC mimics and luciferase reporters containing Circ_0000915-wt or Circ_0000915-mt. E Lysates from HemSCs were incubated with in vitro-synthesized biotin-labeled sense (Oligo probe) or antisense (Circ_0000915 probe) DNA probes against Circ_0000915 for biotin pull-down assay, followed by qRT-PCR analysis to examine Circ_0000915 and miR-890 levels. F, G Expression of miR-890 was measured by qRT-PCR in Circ_0000915-silent (F) and Circ_0000915-overexpressing (G) HemSCs. Data are presented as mean ± S.D from three independent experiments. *P < 0.05; **P < 0.011; ns = not significant. Student’s t-test