Fig. 5

Circ_0000915 up-regulated RNF187 via inhibition of miR-890 in HemSCs. A The prediction for miR-890 binding sites on RNF187 transcripts and schematic of luciferase reporter vector constructs RNF187 3’UTR wild-type (RNF187 3’UTR-wt) and the miR-890-binding-site mutated (RNF187 3’UTR-mt) one. B Biotin-coupled miR-890 (Biotin-miR-890) captured a fold change of RNF187 mRNA in the complex as compared with biotin-coupled miR-NC (Biotin-miR-NC) in biotin-coupled miRNA capture in HemSCs. C, D The luciferase activities in HemSCs and HEK 293t cells co-transfected with miR-890 or miR-NC mimics and luciferase reporters containing RNF187 3’UTR-wt or RNF187 3’UTR-mt. E–G Expression of RNF187 in HemSCs transfected with miR-890 mimics and miR-890 inhibitor or their corresponding negative control was measured by qRT-PCR and Western blot. H–J Expression of RNF187 in Circ_0000915-silent and Circ_0000915-overexpressing HemSCs was measured by qRT-PCR and Western blot. Data are presented as mean ± S.D from three independent experiments. *P < 0.05; **P < 0.011; ns = not significant. Student’s t-test