Fig. 7
Effects of Circ_0000915 on propranolol resistance of HemSCs depended on miR-890/RNF187 pathway. HemSCs were co-transfected with si-NC + NC-inhibitor, si-Circ#1 + NC-inhibitor or si-Circ#1 + miR-890-inhibitor. A RNA levels of miR-890 and RNF187 were examined by qRT-PCR in these co-transfected HemSCs. B Protein level of RNF187 in these co-transfected HemSCs was detected by Western blot. C CCK-8 assay showed cell viability of these co-transfected HemSCs exposed to different concentration of propranolol for 72 h. D IC50 value of propranolol (72 h treatment) was calculated after HemSCs were subjected to the co-transfection. E, F Expression of proliferative-related markers (Cyclin D1 and PCNA) in these co-transfected HemSCs exposed to propranolol (20 μM) for 48 h was measured by qRT-PCR (E) and Western blot (F). G, H Cell apoptosis of these co-transfected HemSCs exposed to propranolol (20 μM) for 48 h were determined by caspase-3 activity assay (G) and Annexin-V/PI double staining assay (H). Data are presented as mean ± S.D from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. (Two-way ANOVA for C, Student’s t-test for others)