Fig. 3

TTC12 knockdown causes defective LR patterning in zebrafish embryos. A Efficiency of ttc12-MO is evaluated by simultaneous coinjection of ttc12 (N-terminal)-eGFP mRNA that effectively abrogates GFP signals in embryos at 16 hpf. Scale bar, 250 μm. B An overview of the morphology of zebrafish embryos injected with Ctrl-MO or ttc12-MO. C A representative image of body curvature and pericardium enlargement in ttc12-MO-treated embryos is shown. Scale bar, 300 μm. D Percentage of body curvature and pericardium enlargement in ttc12-MO- or Ctrl-MO-treated embryos as indicated. E A mild reduction in cilia density was identified in the tail fin (right panels) and pronephros (left panels) of ttc12-MO-treated embryos compared with Ctrls. A representative image is shown. Scale bar, 5 μm. F Representative images of whole-mount immunofluorescence showing inversus, solitus or ambiguous situs in zebrafish embryos by staining cardiac myosin light chain-2 (cmlc2) antibody and DAPI using a confocal microscope at 48 h postfertilization. Scale bar, 100 μm. G Percentage of embryos with situs solitus, situs ambiguous, and situs inversus in the uninjected control (UC) and embryos injected with ttc12-MO or Ctrl-MO