Genotyping of SELE-02 alleles using the MGB Eclipse System of 102 CEPH samples. Real-time curves in the fluorescein channels (Fa) and tetrachlorofluorescein (Fb) are shown in a) and c), respectively. Melt curve analysis in the fluorescein and tetrachlorofluorescein channels are shown in b) and d), respectively. A scatter plot of the real-time data from a) and c) is shown in e). Samples 1 and 2 are outlier samples in the scatter plot, genotyped in b) and d) as unequivocally homogeneous wild-type samples. The x-axis and y-axis in a) and c) represent the cycle number and fluorescent signal in fluorescent units, respectively. The x-axis and y-axis in b) and d) represent the temperature in °C and the first derivative, respectively.