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Table 1 PCR primers, pyrosequencing primers and PCR annealing temperatures used for genotyping

From: Influence of SLCO1B1 and CYP2C8gene polymorphisms on rosiglitazone pharmacokinetics in healthy volunteers

Polymorphism

Primer sequence (5' to 3')

Annealing temperature

SLCO1B1 - 11187 G > A

PCR-forward: TGCTGCAACCATATCAACAAA

PCR-reverse: Biotin-TGGACATTAAGCTCTCTTCTGAAA

Sequencing: ATATATATGTGCATATGTG

60°C

SLCO1B1 -10499 A > C

PCR-forward: Biotin-GTAAAAGCCATTCCCAAAAGTAAG

PCR-reverse: CCACCTTATTATGTCATTGCTCTA

Sequencing: GTTTTAAGAGTTCACTCCAG

60°C

SLCO1B1 388 A > G

PCR-forward: CATTTCACTTTTACCCATC

PCR-reverse: Biotin-TACCTTTTCCCACTATCTCA

Sequencing: ATTCTAAAGAAACTAAT

55°C

SLCO1B1 521 T > C

PCR-forward: Biotin-AGGAATCTGGGTCATACATGTGG

PCR-reverse: CCCCTATTCCACGAAGCATATT

Sequencing: AAGCATATTACCCATGAAC

60°C

CYP2C8*3, Argl39Lys

PCR-forward: Biotin-GGCGTTTCTCCCTCACAACCT

PCR-reverse: GTCACCCACCCTTGGTTTTTCT

Sequencing: CGGTCCTCAATGCTC

60°C

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Human Genomics

ISSN: 1479-7364