Table 1 Primers used in genotyping and real-time PCR
Experiment | Affymetrix transcript cluster IDa | Gene | Description | Direction | Primer |
|---|---|---|---|---|---|
Genotyping | 3930360 | RUNX | Runt-related transcription factor 1 | Forward | 5'-CCTCTGCTGCCAGTAAAGAAG-3' |
Reverse | 5'-Fam-CCGGCCACTTTATCACAGC-3' | ||||
2719656 | CD38 | CD38 molecule | Forward | 5'-CCTGTCCAGATACTTGATACTTTC-3' | |
Reverse | 5'-Fam-AGTCCAGAGTGCTGCCAGT-3' | ||||
3577940 | CLMN | Calmin | Forward | 5'-Fam-TGCCTCGAAGGTACTTAGGAAGT-3' | |
Reverse | 5'-AGAAGTTTGTACGTGGGCTCTTG-3' | ||||
3160175 | VLDLR | Very low density lipoprotein receptor | Forward | 5'-AGCACACTGTACTCCTAATGCCTG-3' | |
Reverse | 5'-Fam-AGGACAGGCATCAGCATTTTC-3' | ||||
2370433 | CACNA1E | Calcium channel, voltage-dependent, R type, alpha 1E subunit | Forward | 5'-Fam-AATGTTGTGTGCTGTCCCAA-3' | |
Reverse | 5'-TTGCATACAGTGGGCACACA-3' | ||||
2991233 | AHR | Aryl hydrocarbon receptor | Forward | 5'-Fam-AGTTGTACAGACAACCTGGAGA-3' | |
Reverse | 5'-CAGTGATGTATGTGGAGAAGTAAT-3' | ||||
2878074 | NRG2 | Neuregulin 2 | Forward | 5'-TTCCGGCCTAAAGAACTAGCTC-3' | |
Reverse | 5'-Fam-AGACTGGCCTGGGATGTGT-3' | ||||
3284596 | PARD3 | Par-3 partitioning defective 3 homolog | Forward | 5'-GCTTTGGACACACTAGTGTCTGC-3' | |
Reverse | 5'-Fam-ATGAGGATCCAGAGCAGGAAA-3' | ||||
3848243 | INSR | Insulin receptor | Forward | 5'-GAAGTCTTTAGATGGCTTCTCTCTG-3' | |
Reverse | 5'-Fam-GCCTTATATTCTAGGTGCTGAGG-3' | ||||
3721010 | IGFBP4 | Insulin-like growth factor binding protein 4 | Forward | 5'-Fam-GGTGGTAGAAAAGTCTTGGCTC-3' | |
Reverse | 5'-GTGTAAGAATGAGGTTAGGGCAA-3' | ||||
Real-time PCR | 3848243 | INSR | Insulin receptor | Forward | 5'-AAGACCATCGACTCGGTGAC-3' |
Reverse | 5'-GGATCGGCGGATTTTTAGAT-3' | ||||
3577940 | CLMN | Calmin | Forward | 5'-GGTTCTTGGGCTGATATGGA-3' | |
Reverse | 5'-GGCCTTGATAGCCTTCCTCT-3' |