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Figure 1 | Human Genomics

Figure 1

From: Development of a cost-effective high-throughput process of microsatellite analysis involving miniaturized multiplexed PCR amplification and automated allele identification

Figure 1

Electropherograms of group I markers. The x-axis represents DNA fragment size in base pairs, and the y-axis represents fluorescence units. (A) Complete spectrum of group I markers labeled with specific fluorescent tags. D5S346 and D7S481 labeled with Ned, Bat26 and D6S262 labeled with Hex, and Bat25 and D3S3623 labeled with Fam. Similarly labeled fragments are distally spaced in size by assay design to accommodate population distribution and possible microsatellite instability. Nonspecific signals are kept at a minimum; however, there is overlap in spectral emission of Hex into Fam, observed as similar stutter peaks with lower signals at Bat26 and D6S262 that are labeled as Fam. (B) Allele identification of Fam-labeled fragments, Bat25 and D3S3623. Background peaks from the spectral overlap of Hex into Fam were excluded from identification due to either low nonspecific signals compared to specific signals as in Bat25 or by filtering out with specified marker and analysis method parameters as in D3S3623. (C) Allele identification of Hex-labeled fragments, Bat26 and D6S262. (D) Allele identification of Ned-labeled fragments, D5S346 and D7S481.

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Human Genomics

ISSN: 1479-7364