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Figure 2 | Human Genomics

Figure 2

From: Development of a cost-effective high-throughput process of microsatellite analysis involving miniaturized multiplexed PCR amplification and automated allele identification

Figure 2

Electropherograms of group II markers. The x-axis represents DNA fragment size in base pairs, and the y-axis represents fluorescence units. (A) Complete spectrum of group II markers labeled with specific fluorescent tags. D9S171 and D17S250 labeled with Ned, D3S1262 and D2S123 labeled with Hex, and D18S61 labeled with Fam. Similar to group I, the spectral overlap of Hex into Fam is observed at D3S1262 and D2S123. (B) Allele identification of Fam-labeled fragment, D18S61. Nonspecific stutters from the spectral overlap of Hex into Fam were excluded from identification by filtering out with specified marker and analysis method parameters. (C) Allele identification of Hex-labeled fragments, D3S1262 and D2S123. (D) Allele identification of Ned labeled fragments, D9S171 and D17S250. Nonspecific peaks outside of marker size range were excluded from identification by filtering out with specified marker and analysis method parameters.

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Human Genomics

ISSN: 1479-7364