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Table 1 Analysis method parameters

From: Development of a cost-effective high-throughput process of microsatellite analysis involving miniaturized multiplexed PCR amplification and automated allele identification

Analysis method

Multiplex PCR group 1

Multiplex PCR group 2

Peak detector tab

  

 Peak detection algorithm

Advanced

Advanced

 Size calling method

Local Southern

Local Southern

 Minimum peak half width

4 points and

2 points

Allele tab

  

 Mono cut-off value

0.95

N/A

 Range filter

  

 Blue

146-194

181-250

 Green

136-159

151-199

 Yellow

116-174

131-174

  1. Optimized parameters for automated specific peak detection, precise sizing, and accurate allele identification.
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Human Genomics

ISSN: 1479-7364