Development of a cost-effective high-throughput process of microsatellite analysis involving miniaturized multiplexed PCR amplification and automated allele identification

Table 2 Marker parameters

Multiplex PCR reaction	Markers	Minimal size	Maximum size	Marker repeat
1	BAT25	115	145	1
1	BAT26	105	135	1
1	D3S3623	200	230	2
1	D5S346	70	115	2
1	D6S262	160	190	2
1	D7S481	175	205	2
2	D2S123	200	240	2
2	D3S1262	120	150	2
2	D9S171	100	130	2
2	D17S250	175	210	2
2	D18S61	145	180	2

Defined size ranges of markers, which include the GDB human genome database population distribution and additional allele sizes observed in our studies for optimal automatic allele identification.

ISSN: 1479-7364