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Figure 3 | Human Genomics

Figure 3

From: A gene-specific non-enhancer sequence is critical for expression from the promoter of the small heat shock protein gene αB-crystallin

Figure 3

The 10-bp sequence functions optimally only in appropriate position and orientation. Schematic representations of various constructions and corresponding activity profiles (RT-qPCR) are shown in (A) and (B). (A) Complete (−896/+44) cryab promoter/tGFP constructs were used. The values shown are the means ± standard deviation. Note that all manipulations of the 10-bp sequence (−64/−55) have a drastic inhibitory effect on tGFP expression. (B) Functional consequences (RT-qPCR) of moving GPS upstream to different distances from the TSS. (C) A plot of the expression of tGFP as a function of the distance of GPS from TSS. Data from three separate experiments (EXP1-3) is plotted. Note that the response is biphasic, and moving GPS to 5' upstream beyond −154 has an inhibitory effect on tGFP expression. The key to various notations is given in the top right box.

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Human Genomics

ISSN: 1479-7364