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Figure 4 | Human Genomics

Figure 4

From: A gene-specific non-enhancer sequence is critical for expression from the promoter of the small heat shock protein gene αB-crystallin

Figure 4

Comparison of point mutations versus deletions on the tGFP expression driven by cryab promoter. Complete promoter constructions were used as in Figures 2 and 3. Expression of tGFP was assessed by RT-qPCR of total RNA. Note that both the point mutations and deletions (in HSE and GPS) have the same relative effect on the tGFP expression (compare numbers 2 and 5 and numbers 3 and 4). Importantly, alterations in the 10-bp sequence have a more pronounced effect on the tGFP expression than alterations in the HSE (compare number 2 with 3 and number 5 with 4). The construction (number 6) contains deletions of both the 10-bp sequence (GPS) as well as the HSE. Interestingly, the effect on activity is similar to that obtained with deletion of the 10-bp sequence (GPS) alone (number 4). The constructs numbers 2 and 3 correspond to constructs numbers 3 and 5, respectively, in Figure 2A. The key to the schematics is given at the bottom.

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Human Genomics

ISSN: 1479-7364