Overview of the experimental procedure. (A) Sample collection at 0, 4, and 8 days post-lentectomy. Lenses were surgically removed from the newt’s eyes and iris rings were split into dorsal-ventral halves before subject to LC-MS/MS. (B) Comparison between newly obtained data and previously described newt transcriptome results. Data were used to validate annotated transcripts at the protein level and identify non-annotated proteins that are probably unique to newts or amphibians.