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Fig. 4 | Human Genomics

Fig. 4

From: Oxidative stress-induced chromosome breaks within the ABL gene: a model for chromosome rearrangement in nasopharyngeal carcinoma

Fig. 4

Nested IPCR detection of DNA breakages within the ABL gene in H2O2-treated NP69. NP69 cells at 30–40% confluency were either untreated (lane 3) or treated with 10 μM (lanes 4 and 7), 50 μM (lanes 5 and 8) or 100 μM (lanes 6 and 9) of H2O2 for 16 h (lanes 4–6) and 24 h (lanes 7–9). Genomic DNA was isolated and manipulated for nested IPCR. In the manipulation for nested IPCR, the DNA samples were subjected to digestion with Age I (a), double digestion with Age I and EcoR I (b) or double digestion with Age I and BsaA I (c). The IPCR products were analysed on 1% agarose gel. Side arrows in panels a and c indicate the position of the 3 kb IPCR bands resulting from the amplification of the intact ABL gene. Side brackets in panels a, b and c indicate the possible IPCR bands from the ABL cleaved fragments. Negative control for PCR was included (lane 10). This IPCR result is representative of 2 repeats with similar results. M1: 1 kb DNA ladder. M2: 100 bp DNA ladder

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