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Fig. 4 | Human Genomics

Fig. 4

From: Downregulation of miR-542-3p promotes osteogenic transition of vascular smooth muscle cells in the aging rat by targeting BMP7

Fig. 4

miR-542-3p regulated osteogenic transition by targeting BMP7. yVSMCs overexpressing miR-542-3p (miR-yVSMCs) were transduced with BMP7-overexpressing lentivirus or control empty virus. After 7 days of induction by β-GP, the mRNA (a) and protein (b) expression levels of smooth muscle markers (SM22α and calponin) and osteogenic markers (OPN and Runx2) were detected. c Quantification of protein expression levels by densitometric analysis. d Calcium deposition detected by Alizarin red staining. e Quantification of calcium deposition. Control: miR-yVSMCs; Vector: miR-yVSMCs transduced with control empty virus; BMP7 overexpression: miR-yVSMCs transduced with BMP7-overexpressing lentivirus. n = 3. Versus control and vector groups, *p < 0.05, **p < 0.01

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