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Fig. 2 | Human Genomics

Fig. 2

From: Aldh1l2 knockout mouse metabolomics links the loss of the mitochondrial folate enzyme to deregulation of a lipid metabolism observed in rare human disorder

Fig. 2

Levels of ALDH1L2-relevant metabolites in the liver of Aldh1l2+/+ and Aldh1l2−/− male mice. a Levels of reduced folate pools (FA, folic acid; THF, tetrahydrofolate; 5-MTHF, 5-methyl-THF; 10-CHO-THF, 10-formyl-THF). For each group, samples from 3 mice were analyzed in quadruplicate. bd Levels of NADPH, NADPH plus NADP+ (NADPt), and the ratio between oxidized/reduced coenzyme. Five and six mice were analyzed for Aldh1l2+/+ (WT) and Aldh1l2−/− (KO) groups, respectively; analysis was done in quadruplicate. e Levels of ATP in mitochondria, cytosol, and total liver lysates. Number of biological replicates (be) correspond to the number of dots; for each sample, measurements were performed in quadruplicate. f Relative changes in ATP between Aldh1l2+/+ and Aldh1l2-/- mice. Numbers in ae show p values (statistical analysis was carried out using graph pad Prism VIII software. Data are expressed as mean ± SE. Statistical significance was calculated using Student’s t test)

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