The transcriptome profile of human trisomy 21 blood cells

Table 2 Genes selected for the comparison of trisomy 21 (T21) vs normal control (n) blood cell transcriptome maps with real-time RT-PCR

Chr	Gene symbol	RNA-Seq T21/n ratio	Real-time T21/n ratio
A
chr15	B2M	1.31	1.29
chr5	DHFR	1.00	0.17
chr21	DYRK1A	1.65	1.01
chr21	ETS2	2.80	2.81
chr12	*GAPDH*	1.21	1.00
chr19	HCST	1.15	0.86
chr11	LRP5	0.66	0.60
chr21	MX1	6.76	6.96
chr9	NACC2	1.15	1.48
chr7	NDUFA4	1.41	1.06
chr17	NPTX1	0.25	0.13
chr11	NRGN	0.87	0.77
chr12	NUAK1	4.47	7.84
chr19	PRDX2	1.05	0.68
chr1	RYR2	1.13	0.37
chr17	SERPINF1	2.33	0.67
chr21	SOD1	1.41	1.33
chr12	TUBA1B	1.13	0.57
chr7	YKT6	1.16	1.15
B
chr10	IFIT1	5.76	0.90
chr21	SLC19A1	3.50	2.02
chr21	TSPEAR	16.79	9.27
chr21	MX1	6.76	2.07
chr21	GART	1.58	2.80
chr12	*GAPDH*	1.21	1.00

A) Genes randomly selected for the validation by real-time RT-PCR of the RNA-Seq results on the same samples used for RNA-Seq. B) Genes with a statistically significant over-expression in the RNA-Seq results, validated by real-time RT-PCR on a larger cohort of samples. From left to right: chromosome, official gene symbol; T21/n expression ratios obtained by TRAM analysis following RNA-Seq data elaboration; T21/n expression ratios determined by real-time RT-PCR. The genes in bold are used as a reference

ISSN: 1479-7364