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Table 1 Overview of plasma input volumes and eluate volumes for each kit and protocol

From: Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA

Kit (purification protocol)

Plasma input volume (mL)

Eluate volume (µl)

MIRA

0.06, 0.2, 0.6

18

CCF

1, 4

18

MAX

0.06, 0.2, 1

20**

MIR

0.06, 0.2

12

MAPss (cfNA ss 2000)

2

24–32***

MAPds (cfNA ds 2000)

2

34–44***

CAT (cfDNA/cfRNA 4000)

2*, 4

17–27

  1. *Diluted to 4 mL with PBS according to instructions of the company (CatchGene), **after vacuum centrifugation, ***after purification with Vivacon columns
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Human Genomics

ISSN: 1479-7364