Table 2 Primer and probe sequences of the dPCR assays (+N is LNA nucleotide)
Target | Assay component | Sequence (5′ to 3′) | Genomic location amplicon* |
|---|---|---|---|
NRGN | Forward primer | GTTTCTGATCTCCGTGTGT | chr11:124747135–124747204 (length: 69 bp) |
Reverse primer | CTTGGACATTCCTCTTTATTGTT | ||
Probe (HEX) | TGTGACTGTGCTGGGTTGGA | ||
CAVIN2 | Forward primer | GCACAGTTTGTTAATATTGTCTTG | chr2:191834469–191834542 (length: 73 bp) |
Reverse primer | CCTGCCTTTAGTATGAACCA | ||
Probe (FAM) | ACT + CTAT + TT + GT + AA + GGTTACTT | ||
AIF1 | Forward primer | AGCGAGAGAAAAGGAAAAGCC | chr6:31616837–31616908 (length: 71 bp) |
Reverse primer | CCTTCAAATCAGGGCAACTCA | ||
Probe (FAM) | CCCCCA + GCCAAGAAAG + CTATC | ||
B2M | Forward primer | GTGGAGCATTCAGACTTGTCT | chr15:44715560–44715658 (length: 98 bp) |
Reverse primer | ACGGCAGGCATACTCATCTT | ||
Probe (HEX) | ACA + CTGAATTCACCCCCACTGA |