Fig. 11

Cell trajectory reconstruction based on regulon activity. A Cell developmental trajectory inference based on regulon activity, four branch points in total. B The direction of pseudotime in trajectory plot for Fig. 9A. C Cell developmental trajectory branch plot based on gene expression. The upper-right trajectory plot indicates the direction of pseudotime. D Cell lineage relationships in Fig. 9A. E Continuum of dynamic target gene expression around branch point 2 in Fig. 9A, D. Cell fate 1 was correlated to the up branch after branch point 2 in Fig. 9A (left branch after branch point 2 in Fig. 9D). Cell fate 2 was correlated to the down branch after branch point 2 in Fig. 9A (right branch after branch point 2 in Fig. 9D). Pixel color indicates the expression levels. All target genes are clustered in 2 groups based on their expression pattern. F NDM of CREM, FOSL2, FOXC2, RUNX2, and CREB3L1 TFs. Stars indicate the significance levels of the NDM difference from any other cell clusters (adjusted p value < 0.05, Wilcoxon rank-sum test)