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Fig. 13 | Human Genomics

Fig. 13

From: Regulon active landscape reveals cell development and functional state changes of human primary osteoblasts in vivo

Fig. 13

Experimental validation in MC3T3-E1 preosteoblastic cell line. A Expression of Crem, Fosl2, Foxc2, Runx2 and Creb3l1 genes during osteogenic differentiation process in 0, 2, 3, 7 and 14 days. B Alizarin Red S staining at 0 and 14 days. C Representative confocal immunofluorescent imaging showing distribution of Foxc2+Alp+ osteoblast in murine femur. Arrows mark Foxc2+Alp+ cells. D Qpcr experiment revealed the successful knockdown of Foxc2. E Gene expression of Runx2, Col, Alp and Osx at 3 days. F Gene expression of Runx2, Col, Alp and Osx at 7 days. (*, P < 0.05 by one-way ANOVA; ns not significant, OM osteogenic differentiation medium)

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Human Genomics

ISSN: 1479-7364