Fig. 2

Single-cell clustering and regulatory network inference. A Gene expression-based cell clustering results shown using the Seurat clustering layout. Marked out three cell clusters. B Cell developmental trajectory inference based on gene expression. Marked three cell clusters. The upper-right trajectory plot indicates the direction of pseudotime. C Expression levels (log-normalized) of indicated genes in the three osteoblast clusters with respect to their pseudotime coordinates. The x-axis indicates the pseudotime, while the y-axis represents the log-normalized gene expression levels. Black lines depict the LOESS regression fit of the normalized expression values. D Gene expression and TF regulatory network conjoint clustering results shown using the SCENIC clustering layout. Marked out four cell clusters. E Regulons active heatmap in four cell clusters