Fig. 5

Dynamic changes of FOSL2 regulon activity. A FOSL2 regulatory network. Red dots represent immunity-related target genes, blue dots represent cell proliferation/differentiation-related target genes, yellow dots represent both immunity and cell proliferation/differentiation-related target genes; other target genes are shown as gray dots. B FOSL2 regulon activity, embedded on SCENIC clustering layout and colored by FOSL2 regulon active levels. Gray dots represent completely inactive cells with zero active scores. Other dot colors represent active score in each cell from black (low) to blue (high). C Violin plots showing expression levels of FOSL2. *Adjusted p value < 0.05 (Kruskal–Wallis test). D Violin plots showing average expression levels of all target genes in FOSL2 regulon. *Adjusted p value < 0.05 (Kruskal–Wallis test). E Immunity and cell proliferation/differentiation-related target genes expression in FOSL2 regulon among four cell clusters. Dot color indicates the relative expression levels and dot size shows the proportion of cells expressing the indicated genes. F Continuum of dynamic target gene expression in pseudotime of osteoblasts. Pixel color indicates expression levels. Early-stage cells are listed on the left. G Motif cisbp_M3083 of FOSL2. The relative sizes of the letters indicate the frequency of four bases in the sequences