Fig. 7

Dynamic changes of MXD4 and KLF2 regulons activity. A MXD4 regulatory network. Target genes are shown as gray dots. B KLF2 regulatory network. Target genes are shown as gray dots. C MXD4 and KLF2 regulon activity, embedded on SCENIC clustering layout and colored by MXD4 or KLF2 regulon active levels. Gray dots represent completely inactive cells with zero active scores. Other dot colors represent active scores in each cell from black (low) to blue (high). D Violin plots showing expression levels of MXD4 (left) and KLF2 (right). *Adjusted p value < 0.05 (Kruskal–Wallis test). E Violin plots showing average expression levels of all target genes in MXD4 regulons. *Adjusted p value < 0.05 (Kruskal–Wallis test). F Continuum of dynamic MXD4 and KLF2 regulon’s target gene expression in pseudotime of osteoblasts. Pixel color indicates expression levels. Early-stage cells are list at left. G Motif hocomoco__USF2_HUMAN.H11MO.0.A of MXD4. H Motif transfac_pro__M07913 of KLF2. The relative sizes of the letters indicate the frequency of four bases in the sequences