Fig. 8

Dynamic changes of FOXC2 and TAF7 regulon activity. A FOXC2 regulatory network. Blue dots represent MSC differentiation-related target genes; other target genes are shown as gray dots. B TAF7 regulatory network. Target genes are shown as gray dots. C FOXC2 and TAF7 regulon activity, embedded on SCENIC clustering layout and colored by FOXC2 or TAF7 regulon active levels. Gray dots represent completely inactive cells with zero active scores. Other dot colors represent active scores in each cell from black (low) to blue (high). D Violin plots showing expression levels of FOXC2 (left) and TAF7 (right). *Adjusted p value < 0.05 (Kruskal–Wallis test). E MSC differentiation-related target gene expression in FOXC2 regulon among four cell clusters. Dot color indicates relative expression levels and the dot size shows the proportion of cells expressing the indicated genes. F Violin plots showing average expression levels of all target genes in FOXC2 regulons. *Adjusted p value < 0.05 (Kruskal–Wallis test). G Continuum of dynamic FOXC2 and TAF7 regulon target gene expression in pseudotime of osteoblasts. Pixel color indicates expression levels. Early-stage cells are list at left. H Motif hocomoco__FOXL2_MOUSE.H11MO.0.C of FOXC2. I Motif dbcorrdb__TAF7__ENCSR000BLU_1__m1 of TAF7. The relative sizes of the letters indicate the frequency of four bases in the sequences