Fig. 3
Gene expression profiles in the brain in Tsc1+/−, Tsc2+/−, and TscD+/− mice. A Histograms of the log2 (fold-change) (n = 45,313, bin width = 0.02) for each mutant mouse compared with WT (cyan: Tsc1+/− vs. WT, magenta: Tsc2+/− vs. WT, blue: TscD+/− vs. WT). Solid lines represent approximate normal distribution curves. SD standard deviation. B Venn diagram of three DETs between mutant and WT mice. Among overlapping transcripts (yellow area), transcripts that changed in different directions (▲: upregulated, ▼: downregulated) were excluded from each entity for further analysis. C Heat map of valid 1119 transcript profiles in vehicle-treated samples for four genotypes (n = 4 for each genotype) that were in common with at least two DETs and met the criteria. Hierarchical clustering was based on the relative expression value after baseline correction to the median of vehicle-treated WT samples (red: upregulated, blue: downregulated). D Top 20 rank map folders enriched for differentially expressed 1119 transcripts in mutant mice. The blue and red folders indicate cancer/neoplasm and immunity/inflammation, respectively. FDR false discovery rate