Fig. 4
Rapamycin-induced changes in gene expression in the brain in Tsc1+/−, Tsc2+/−, and TscD+/− mice. A The cyan-colored circle indicates transcripts whose expression significantly changed in Tsc1+/− mice and was reversed by treatment with rapamycin ([Tsc1+/− vs. WT] ∩ [Tsc1+/− Veh vs. Rap]). Magenta- and blue-colored circles indicate gene expression changes in Tsc2+/− and TscD+/− mice, respectively. Canonical pathway modeling analysis for these three groups of transcripts identified seven, five, and six significant networks in Tsc1+/−, Tsc2+/−, and TscD+/− mice, respectively (p < 0.05, zScore > 100). B Box-and-whisker plot of normalized log2 signal intensity for the gene expression of Pdlim2 in vehicle- or rapamycin-treated mutant and WT mice. **p < 0.01; ***p < 0.001 (two-way ANOVA). Veh vehicle, Rap rapamycin