Table 9 Primer sequences for APP gene SNP genotyping and methylation detection

rs2040273

Amplify upstream primers: 5'-ACGTTGGATGGAGGAATGTAGTAGACCGAC-3'

Amplify downstream primers: 5'-ACGTTGGATGCTCCATAACCAAACCAAACC-3'

Single-base extension primer: 5'-CCAAACCCTTTCATATCATTT-3'

rs63750921

Amplify upstream primers: 5'-ACGTTGGATGGTGGGTTCAAACAAAGGTGC-3'

Amplify downstream primers: 5'-ACGTTGGATGCAAGGTGATGACGATCACTG-3'

Single-base extension primer: 5'-ggacCACCGCCCACCATGA-3'

rs2211772

Amplify upstream primers: 5'-ACGTTGGATGAGGGAGGAAATGACAGAGAG-3'

Amplify downstream primers: 5'-ACGTTGGATGGTGCAAGTAGGTTGGATCTC-3'

Single-base extension primer: 5'-aCTCATGATAGTCCATATTCAC-3'

rs2830077

Amplify upstream primers: 5'-ACGTTGGATGTGTCTCCTTATGGAGAGTGG-3'

Amplify downstream primers: 5'-ACGTTGGATGAGTGTCTCTCTGAAGTGGTG-3'

Single-base extension primer: 5'-cccctGTGACACGCTGACTTCCAGGCA-3'

rs467021

Amplify upstream primers: 5'-ACGTTGGATGGCATTGGTGCTTGTAATATC-3'

Amplify downstream primers: 5'-ACGTTGGATGCTGCTTTCTTCTGACTTACC-3'

Single-base extension primer: 5'-aACTTACCATGAGAATTCCA-3'

rs368196

Amplify upstream primers: 5'-ACGTTGGATGTTTCTTCCTCCACTGGACTG-3'

Amplify downstream primers: 5'-ACGTTGGATGAGATCTAGAATCTGGGTGGG-3'

Single-base extension primer: 5'-GGCTGTGAGTAAATAGAAAGGTA-3'

rs466433

Amplify upstream primers: 5'-ACGTTGGATGGATCATTCGTATTCGACCCC-3'

Amplify downstream primers: 5'-ACGTTGGATGTCAGGACAGACACAATGAAG-3'

Single-base extension primer: 5'-AACAAGGGCAGCGTT-3'

rs364048

Amplify upstream primers: 5'-ACGTTGGATGTTCTGCCATGCCACTTTCTC-3'

Amplify downstream primers: 5'-ACGTTGGATGTGGGCAGTTCTAGAGCATTC-3'

Single-base extension primer: 5'-gacttGACAGTGGACGGTTTGTGTTT-3'

rs364051

Amplify upstream primers: 5'-ACGTTGGATGTGCACTGCAGCCTGCCTTC-3'

Amplify downstream primers: 5'-ACGTTGGATGAGGAAGGAAGTCTGTACCCC-3'

Single-base extension primer: 5'-GTCAGCGCAATGAGCA-3'

rs438031

Amplify upstream primers: 5'-ACGTTGGATGCCCCATCCTAGTTTCAAGTG-3'

Amplify downstream primers: 5'-ACGTTGGATGCCAGAAATGCCCAAAGATAG-3'

Single-base extension primer: 5'-cagcAATGCCCAAAGATAGAATGCAC-3'

rs463946

Amplify upstream primers: 5'-ACGTTGGATGACTGTTGAAGGAAGTGCCTG-3'

Amplify downstream primers: 5'-ACGTTGGATGCAAATTTGCCAGCGGTTTTC-3'

Single-base extension primer: 5'-agcgtTTTTCATGCTACTTCTTCCT-3'

Methylation detection

Upstream primers: 5'-aggaagagagGGTTTGGTATTGTTTTTGTTGGT-3'

Downstream primers: 5'-cagtaatacgactcactatagggagaaggctAAAAACTCCTAACTTCCTAAACTATCC-3'