In silico prioritisation of microRNA-associated common variants in multiple sclerosis

Table 1 Annotation of the 6 miRNA SNPs that passed the Bonferroni-adjusted p value threshold (p < 0.05/314)

CHR: POS (hg38)	RSID	A1/A2	EUR_AF	Discovery GWAS p value	OR	miRNA	ADmiRE annotations	OR interpretation	Prioritisation results
1:116560027	rs1414273	C/T	0.1402	8.48E−16	1.2333	mir-548ac	Precursor_3PrimeEnd	risk	High LD, structural change
8:128150187	rs2648841	G/A,T	0.0179	3.86E−05	1.1322	mir-1208	Precursor_3PrimeEnd	risk	No structural change
6:31355288	rs17881225	G/C	0.0984	7.72E−05	1.2163	mir-6891	Precursor_Loop	risk	Signal in high LD HLA region
6:31355243	rs2276448	T/C	0.2366	7.73E−12	1.2517	mir-6891	3p_Seed	risk	Signal in high LD HLA region
6:31355235	rs2854001	G/A	0.2117	5.44E−38	1.5888	mir-6891	3p_Mature	risk	Signal in high LD HLA region
6:32749925	rs4285314	G/A	0.5318	7.1E−122	1.5134	mir-3135b	Precursor_3PrimeEnd	risk	Signal in high LD HLA region

ADmiRE [28] was used to identify the locations of these SNPs. In order of consequence, SNPs in the seed region > mature > loop > precursor ends. The association with MIR548AC was explored in the previous section. The structural consequence of MIR1208 SNP was explored, while the microRNA-binding ability of MIR6891 was examined in the context of the seed SNP. Discovery GWAS p values and ORs of these SNPs are also presented in context

ISSN: 1479-7364