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Fig. 4 | Human Genomics

Fig. 4

From: A novel upregulated hsa_circ_0032746 regulates the oncogenesis of esophageal squamous cell carcinoma by regulating miR-4270/MCM3 axis

Fig. 4

hsa_circ_0032746 serves as miR-4270 sponge. a The MREs of hsa_circ_0032746. b The binding sites for miR-4270 and hsa_circ_0032746. c The expression level of miR4270 in TE-1 and KYSE 30 cell lines. d The expression level of miR4270 in TE-1 and KYSE 30 cell lines after siRNA transfection. e Prediction of combined sequence of hsa_circ_0032746 WT and miR‐4270 and construction of hsa_circ_0032746 MUT sequence. The luciferase intensity decreased after cotransfection with hsa_circ_0032746 wild-type and hsa- miR-4270 mimics in both cell lines. f Transwell assay illustrated that lentivirus transfection induced knockdown of hsa_circ_0032746 in both cell lines whereas cotransfection with miR-4270 inhibitor reversed the effect. g Colony formation assay showed that sh-circ_0032746 and miR-4270 mimic inhibited the cell proliferation

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Human Genomics

ISSN: 1479-7364