Table 1 Primer sequences and the amplicon size for PCR reaction
Primers | Primer sequences | Product size | Genotyping technique |
|---|---|---|---|
MTHFR-1 | 5′-GCCTCTCCTGACTGTCATCC (Exonic) | 282 bp | RFLP and sequencing |
MTHFR-2 | 5′AGGACGGTGCGGTGAGAGTG (Intronic) |  |  |
MTHFR-3 | 5′-GAAGGAGAAGGTGTCTGCG (Exonic) | 45 bp | HRM |