Table 2 Comparison of plasma biomarker performances in detecting different CYP2D6 phenotypes versus normal metabolizers (n = 196)
From: Solanidine is a sensitive and specific dietary biomarker for CYP2D6 activity
CYP2D6 biomarker | Mean AUPRC (95% CI) | F1max | Mean difference from solanidine (95% CI) | Mean AUROC (95% CI) | Difference from solanidine | |
|---|---|---|---|---|---|---|
Mean (95% CI) | P value | |||||
Poor metabolizer (n = 9) baseline AUPRC = 0.0439 and baseline AUROC = 0.5 | ||||||
Solanidine | 0.730 (0.383, 0.922) | 0.667 | 0.976 (0.944, 0.992) | |||
m/z 414 to solanidine ratio | 1.000 | 1.000 | 0.270 (0.0853, 0.583)* | 1.000 (0.982, 1.000) | 0.0241 (− 0.00503, 0.0532) | 0.105 |
m/z 416 to solanidine ratio | 1.000 | 1.000 | 0.270 (0.0853, 0.583)* | 1.000 (0.982, 1.000) | 0.0241 (− 0.00503, 0.0532) | 0.105 |
m/z 444 to solanidine ratio | 1.000 | 1.000 | 0.270 (0.0853, 0.583)* | 1.000 (0.982, 1.000) | 0.0241 (− 0.00503, 0.0532) | 0.105 |
m/z 430 to solanidine ratio | 1.000 | 1.000 | 0.270 (0.0853, 0.583)* | 1.000 (0.982, 1.000) | 0.0241 (− 0.00503, 0.0532) | 0.105 |
m/z 412 to solanidine ratio | 1.000 | 1.000 | 0.270 (0.0853, 0.583)* | 1.000 (0.982, 1.000) | 0.0241 (− 0.00503, 0.0532) | 0.105 |
Intermediate metabolizer (n = 89) baseline AUPRC = 0.312 and baseline AUROC = 0.5 | ||||||
Solanidine | 0.444 (0.344, 0.548) | 0.488 | 0.610 (0.550, 0.667) | |||
m/z 414 to solanidine ratio | 0.477 (0.376, 0.580) | 0.522 | 0.0333 (-0.123, 0.185) | 0.652 (0.594, 0.707) | 0.0423 (− 0.0387, 0.123) | 0.306 |
m/z 416 to solanidine ratio | 0.504 (0.402, 0.606) | 0.547 | 0.0604 (-0.0909, 0.204) | 0.662 (0.604, 0.717) | 0.0525 (− 0.0131, 0.118) | 0.117 |
m/z 444 to solanidine ratio | 0.620 (0.515, 0.714) | 0.593 | 0.176 (0.0263, 0.316)* | 0.741 (0.686, 0,791) | 0.131 (0.0729, 0.189) | < 0.0001* |
m/z 430 to solanidine ratio | 0.628 (0.523, 0.722) | 0.606 | 0.184 (0.0321, 0.327)* | 0.764 (0.710, 0.812) | 0.154 (0.0903, 0.218) | < 0.0001* |
m/z 412 to solanidine ratio | 0.519 (0.416, 0.621) | 0.524 | 0.0753 (− 0.0872, 0.226) | 0.666 (0.608, 0.720) | 0.0562 (− 0.0171, 0.129) | 0.133 |
Ultrarapid metabolizer (n = 20) baseline AUPRC = 0.0926 and baseline AUROC = 0.5 | ||||||
Solanidine | 0.139 (0.0434, 0.364) | 0.305 | 0.636 (0.568, 0.701) | |||
m/z 414 to solanidine ratio | 0.104 (0.0271, 0.328) | 0.194 | − 0.0343 (0.0258, − 0.105) | 0.524 (0.455, 0.592) | − 0.112 (0.0913, − 0.316) | 0.280 |
m/z 416 to solanidine ratio | 0.195 (0.0744, 0.423) | 0.218 | 0.0566 (− 0.0836, 0.266) | 0.610 (0.542, 0.676) | − 0.0263 (0.108, − 0.161) | 0.702 |
m/z 444 to solanidine ratio | 0.295 (0.138, 0.522) | 0.333 | 0.156 (− 0.0345, 0.391) | 0.689 (0.622, 0.750) | 0.0520 (− 0.0700, 0.174) | 0.403 |
m/z 430 to solanidine ratio | 0.296 (0.138, 0.523) | 0.364 | 0.157 (− 0.0306, 0.400) | 0.741 (0.678, 0.798) | 0.105 (− 0.0384, 0.248) | 0.152 |
m/z 412 to solanidine ratio | 0.144 (0.0462, 0.370) | 0.203 | 0.00553 (− 0.0969, 0.216) | 0.551 (0.482, 0.619) | − 0.0851 (0.0731, − 0.243) | 0.292 |