Fig. 3
From: Mutations in TSPAN12 gene causing familial exudative vitreoretinopathy
Vector construction and analysis of TSPAN12 expression. A Sequencing results showed successful introduction of mutation c.496_612del (-) into recombinant plasmids. B Expression of mut and wt TSPAN12 proteins was examined by Western blot, and the result showed that the protein translated by the cells transfected with the mut vector was significantly lower than proteins which were translated by the cells transfected with the WT plasmid. C The mutation c.612+1G>A cause exon 7 skipping