Fig. 4
From: GEN1 as a risk factor for human congenital anomalies of the kidney and urinary tract
PCR product electrophoresis and sequencing results. PCR substances of shear site variation and wild-type plasmid transcripts were used to identify the difference between the two transcripts. Shear site variation formed shorter exons 10. The sequencing results further confirmed the missing 30 bp sequence. (A, B) Simple schematic diagrams of mRNA formed by WT and Gen1 (c.1071 + 3(IVS10) A > G) mutant proteins. (C) Electrophoresis of PCR products. Lane 1 is the wild type, lane 2 is the shear variant, and lane 3 is the marker. (D, E) Sanger sequencing of PCR products. Abbreviations: MT, mutant type; WT, wild type