- Primary research
- Open Access
Contribution of 3D genome topological domains to genetic risk of cancers: a genome-wide computational study
Human Genomics volume 16, Article number: 2 (2022)
Genome-wide association studies have identified statistical associations between various diseases, including cancers, and a large number of single-nucleotide polymorphisms (SNPs). However, they provide no direct explanation of the mechanisms underlying the association. Based on the recent discovery that changes in three-dimensional genome organization may have functional consequences on gene regulation favoring diseases, we investigated systematically the genome-wide distribution of disease-associated SNPs with respect to a specific feature of 3D genome organization: topologically associating domains (TADs) and their borders.
For each of 449 diseases, we tested whether the associated SNPs are present in TAD borders more often than observed by chance, where chance (i.e., the null model in statistical terms) corresponds to the same number of pointwise loci drawn at random either in the entire genome, or in the entire set of disease-associated SNPs listed in the GWAS catalog. Our analysis shows that a fraction of diseases displays such a preferential localization of their risk loci. Moreover, cancers are relatively more frequent among these diseases, and this predominance is generally enhanced when considering only intergenic SNPs. The structure of SNP-based diseasome networks confirms that localization of risk loci in TAD borders differs between cancers and non-cancer diseases. Furthermore, different TAD border enrichments are observed in embryonic stem cells and differentiated cells, consistent with changes in topological domains along embryogenesis and delineating their contribution to disease risk.
Our results suggest that, for certain diseases, part of the genetic risk lies in a local genetic variation affecting the genome partitioning in topologically insulated domains. Investigating this possible contribution to genetic risk is particularly relevant in cancers. This study thus opens a way of interpreting genome-wide association studies, by distinguishing two types of disease-associated SNPs: one with an effect on an individual gene, the other acting in interplay with 3D genome organization.
Genome-wide association studies (GWAS) have compared the genomes of large cohorts of patients and healthy individuals and evidenced statistical associations between the presence of single-nucleotide polymorphisms (SNPs) in their variant form, and the presence of diseases [1, 2], including cancers [3, 4]. Remarkably, only a few percent of disease-associated SNPs (daSNPs) are located in coding regions of the genome [5, 6]. How the vast majority of non-coding daSNPs are mechanistically related to the risk of developing a disease is yet unclear. While SNPs were at first considered as mere markers of the nearest gene, it rapidly appeared that they can have a direct functional role in affecting the regulation of neighboring genes, typically by being located in regulatory sequences [6, 7]. Systematic analyses of SNP location with respect to genome annotations such as binding sites of regulatory proteins or histone epigenetic marks correlated with promoter or enhancer loci [8, 9], as well as joint transcriptome analysis , have been successfully used to identify causal variants and affected genes.
However, such correlation analyses are not sufficient to unravel all the determinants of SNP-disease associations. An additional ingredient, the three-dimensional (3D) genome organization, must be taken into account. Indeed, it is now acknowledged that not only the adjacent sequences of the gene, but also the 3D folding of the genome play a role in genomic functions [11, 12], and more specifically in the regulation of gene expression  and its mis-regulation, e.g., in cancers . It thus appears essential to reformulate the notion of genetic risk to a disease in the context of the recently acquired knowledge about 3D genome organization. Our working hypothesis is that certain non-coding SNPs could, in their variant form, affect the 3D genome architecture and its role in gene regulation, thus favoring the development of diseases.
This idea has been documented experimentally for enhancer-promoter loops . A variant at a single SNP may induce a change in the looping bringing enhancer and promoter into close spatial proximity, henceforth affecting the expression of the corresponding genes. For instance, a single SNP may modify a CTCF binding site and in turn nucleosome positioning and chromatin 3D architecture, a documented situation for asthma risk [16, 17]. At the MYB locus, 3C experiments have shown reduced interactions between promoter and enhancer in the presence of the at-risk allele, providing an instance of a SNP having a causal architectural effect .
Pursuing this line of investigation at the genome-wide level, we will consider another feature of 3D genome structure, namely topologically associating domains, TADs . There are few genomic contacts between two adjacent TADs, and the insulating capacity of TAD borders  has been shown to be essential for proper gene regulation, by preventing spurious interactions between genes and enhancers located in adjacent TADs [21,22,23]. Gene mis-regulation can occur due to TAD border disruption induced by the presence of short tandem repeats . The importance of TADs and the effect of TAD border disruption (dashed arrow in Fig. 1) have also been shown in the case of Hox genes , or as a way to control developmental genes in drosophila embryos, as we recently proposed . An effect of TAD border disruption has also been observed in cancer cells as a consequence of cancerous mutations [27, 28].
The guideline of our computational study is that functional mechanisms underlying the association of risk loci with a disease may, in some cases, be mediated by a change occurring in TAD borders when embedded SNPs are in their at-risk-form. Accordingly, the increased disease risk due to the presence of non-coding SNPs in their variant form could presumably be better understood by investigating their location with respect to various features of the 3D genome organization, in particular its partitioning into TADs .
We studied quantitatively and systematically, for each disease, the location of disease-associated SNPs with respect to TAD borders (Fig. 1). Different cell types and data obtained in different laboratories have been considered (overall 15 datasets). Based on a preliminary analysis  and on their different etiology, we analyzed separately 71 cancers and 378 non-cancer diseases and compared the distribution of their associated SNPs with respect to TAD borders. We analyzed, for each disease, the distribution of its disease-associated SNPs in TAD borders compared to chance, where chance corresponds to the same number of pointwise loci drawn at random either in the entire genome or in the set of SNPs listed in the GWAS catalog. We investigated whether the results persist when considering only intergenic SNPs (40% of the total set of daSNPs, overall comprising 21,183 entries). An integrated pipeline, sketched in Additional file 1: Fig. S1, has been devised for this analysis, and its different elements are detailed in the Methods section.
We emphasize that the present investigation does not consider somatic mutations appearing in cancer cells along cancer progression. It focuses on genomic variations observed from birth in the genome of any healthy cell of the individual.
For a fraction of diseases, the associated SNPs are preferentially located in TAD borders
We used 15 high-resolution published Hi-C datasets, obtained in two different laboratories, for different human cell types and using different restriction enzymes. We first determined the 3D genome organization in topological domains (TADs) using TopDom algorithm. Then, for each of the 449 diseases considered, the potential over-representation of its associated SNPs in TAD borders (overall covering between about 300 to 500 Mb) has been assessed using a hypergeometric test (see Methods).
Results are shown in Fig. 2A in the form of a p-value histogram. The p-value for a given disease measures the statistical significance of the preferential location of its associated SNPs in TAD borders, or equivalently, the statistical significance of TAD-border enrichment in its associated SNPs. Given the overwhelming number (378) of non-cancer diseases compared to cancers (71) present in the GWAS catalog, and their different etiology, we considered separately cancers and non-cancer diseases.
Our analysis shows that for a fraction of diseases, their associated SNPs are preferentially located in TAD borders, as already observed in a preliminary study . A recent study centered on complex trait heritability consistently put forward a specific status of TAD borders . In our results, the fact that only a fraction of diseases display such a TAD-border enrichment precludes a trivial explanation related to some confounding feature of the TAD borders (e.g., gene density, enhancer location, density of binding sites for architectural proteins, replication origins), that would produce enrichment for all diseases. As an illustration, we give in Additional file 1: File S1 the lists of cancers displaying TAD-border enrichment in their associated SNPs, in the case of IMR90 and NHEK cell lines.
Despite the well-known variability in TAD determination [33,34,35] (see Methods and Additional file 1: Fig. S2, Additional file 1: Fig. S3), we get a clear statistical result, both for a single value of the parameter k (window size) of the TAD caller TopDom, or when aggregating over several values of this parameter.
TAD-border enrichment in daSNPs is still observed with intergenic SNPs alone
To better interpret the over-representation of daSNPs in TAD borders, we considered specific subsets of daSNPs according to their genomic location: namely daSNPs located in exons (5%), whose variant form has an impact on a protein sequence (coding SNPs) and those, by far more common, located either in introns (55%) or in intergenic regions (40%). We then performed the enrichment analysis considering only intergenic daSNPs. Figure 2B shows that TAD-border enrichment is still observed and even enhanced for intergenic SNPs.
Preferential location of daSNPs in TAD borders is observed mainly for cancers
The normalized histograms presented in Fig. 2A indicate that the fraction of cancers displaying TAD-border enrichment in daSNPs is larger than the fraction of non-cancer diseases displaying such a preferential location of their associated SNPs. To better evidence this relative predominance of cancers, we present on Fig. 2C the difference between the two histograms. Considering a percentage among cancers is motivated by the small number of cancers (71) in the whole set of diseases (449 EFOs listed in the GWAS). An alternative and equivalent formulation is that the fraction of cancers among the diseases displaying enrichment is larger than the fraction of cancers among the overall set of diseases.
This observation motivated a systematic quantification of the relative predominance of cancers, presented in Fig. 3 together with an assessment of its statistical significance. Similar results have been obtained for the genome-based and the SNP-based null models (see Methods), as expected from the comparison of the enrichment p-values obtained with these two null models, presented in Additional file 1: Fig. S4.
We analyzed further the robustness of this relative predominance of cancers among the diseases displaying TAD-border enrichment. We observed that it is sensitive but overall robust with respect to the parameter k of the TAD caller (Additional file 1: Fig. S2). This relative predominance of cancers is equally observed before correcting for multiple testing or using a global correction (see Additional file 1: Fig. S5), demonstrating that it is not an artifact of applying the correction separately for cancers and non-cancer diseases.
The relative predominance of cancers among diseases displaying TAD-border enrichment is enhanced when only intergenic SNPs are considered
We then dissected the contribution of exonic, intronic and intergenic SNPs to TAD-border enrichment. Mainly, the relative predominance of cancers among diseases displaying TAD-border enrichment in intergenic daSNP is confirmed and even enhanced compared to the quantification involving all types of daSNPs (Figs. 2D, 3A). The same shift is observed for both null models (data not shown, but see Additional file 1: Fig. S4), and it is robust with respect to the type of multiple-correction adopted (see Additional file 1: Fig. S6).
That exonic SNPs also contribute to TAD-border enrichment in daSNPs is not surprising nor contradictory, as TAD borders are known to host active genes [19, 39]. We actually observe a stronger and unexpected feature, namely a relative dominance of non-cancer diseases when restricting to exonic daSNPs. However, the low number (a few units) of exonic daSNPs for most diseases brings statistics to a limit and precludes elaborating too much on this observation. In particular, although visible in all cases, the relative dominance of non-cancer diseases reaches statistical significance only in the aggregated data. This observation nevertheless confirms the specificity of cancers, compared to non-cancer diseases, regarding the role of TADs in the associated genetic susceptibility.
Different enrichments are observed in embryonic stem cells (hESC)
We consistently observe the above-described results for most cell types, as shown in Fig. 3B–G and Additional file 1: Fig. S7. The relative dominance of cancers among diseases displaying TAD-border enrichment in their associated intergenic SNPs largely reaches statistical significance, with a few notable exceptions: umbilical vein cells (HUVEC, Fig. 3G), embryonic stem cells (hESC, Additional file 1: Fig. S7A, E), H1-derived cells (H1_ME, H1_NP, H1_TB, Additional file 1: Fig. S7F, G, H).
We further assess the different enrichment results between embryonic stem cells and differentiated cell types in a systematic comparison of hESC and IMR90 cell lines, for which several datasets from different studies are available, Fig. 4. Consistent results are obtained for the two datasets obtained in hESC (Fig. 4, blue panels), and the three datasets for IMR90 (Fig. 4, pink panels), respectively. These results can be extracted more clearly by an aggregation over datasets for the same cell type (Fig. 4, top panels). The comparison emphasizes the peculiarity of hESC, with no relative predominance of cancers among diseases displaying TAD-border enrichment in intergenic daSNPs. In H1 hESC and derived cells at early developmental stage (H1_ME, H1_NP, H1_TB), 3D genome partitioning in TADs is not significantly related to the location of cancer risk loci. Consistently, H1_MS cell line differs from the other H1-derived cell lines both in our analysis and in the original study , which has evidenced a genome-wide evolution of TAD structure in this series of H1-derived cell lines following their progressive differentiation. We also observe that HUVEC line has the same signature as hESC, with no cancer-specific features in the location of at-risk SNPs with respect to the TAD structure present in this cell line.
Our analysis thus suggests that TAD-border contribution to the genetic risk of cancers is not observable in HUVEC, hESC and derived cells at early developmental stage (H1_ME, H1_NP, H1_TB) possibly due to their different (not fully mature) TAD structure [38, 40].
SNP-based diseasome analysis shows that TAD-border enrichment features are not due to a few daSNPs common to several diseases
Among the disease-associated SNPs listed in the GWAS catalog, about 14% are actually associated with several diseases. To analyze the influence of such events on our results, we devise a network representation, where the nodes of the network are diseases (coloring differently cancers and non-cancer diseases) and a link is drawn between two diseases when they share at least one associated SNP. This is nothing but the SNP-based analog of the diseasome networks introduced in , in which a link is drawn between two diseases when they share a related gene. We then compared the networks obtained when a link is drawn only when the diseases share a border SNP, i.e., a SNP located in a TAD border for a majority of values of the window parameter (Fig. 5A), when the diseases share a SNP belonging to the complementary set (non-border SNPs, Fig. 5B), then with an additional filter keeping only intergenic SNPs (Fig. 5C, D).
We observe on Fig. 5 that cancers share SNPs preferentially with other cancers, corresponding in the network language to an assortative clustering of cancer nodes. Similar clustering properties are visually observed in the networks based on border SNPs or intergenic border SNPs.
As the visual inspection of the networks can be misleading, we quantified for various node subsets the statistical enrichment of links of each induced subgraph. For this purpose, we used an indicator termed the network coherence [42, 43]. Network coherence basically assesses whether the nodes in the set under consideration are more (or less) connected to each other than expected at random. It is defined as a z-score (see Methods) so as to get absolute values than can be used for comparing networks. A random subgraph would have a vanishing network coherence, while a positive value indicates a significantly higher number of internal links compared to random sets of the same size. Additional file 1: Table S1 lists all network coherences for all four networks and all six node types: cancer or non-cancer, displaying TAD-border enrichment (termed in short ‘enriched’) or not.
For non-cancer diseases, the enrichment status makes a difference: The passage from not-enriched to enriched leads to a change of sign (from negative to positive) in the network coherence (from − 1.50 to + 2.09, in the case of border SNPs), suggesting a more important contribution of specific shared SNPs to TAD-border enrichment in the class of non-cancer diseases.
For cancers, the enrichment status does not make a difference: Both subsets of cancers (not-enriched and enriched) have a high network coherence (+ 3.20 and + 2.94, in the case of border SNPs) and, hence, qualitatively speaking, display similar overlaps among SNP sets, either border SNPs or non-border SNPs. The presence of SNPs associated with multiple cancers therefore has a similar impact on our analysis both for cancers displaying TAD-border enrichment and for other cancers. Moreover, the effect becomes weaker when imposing the additional filter of intergenic SNPs. Therefore, our statistical observations regarding TAD-border enrichment in cancer-associated SNPs do not arise from a few shared border SNPs but actually from their preferential location in TAD borders.
We have explored the notion of genetic risk to a disease in the context of the recently acquired knowledge about 3D genome organization, specifically the genome partitioning into topological domains (TADs). We have provided statistical evidence that for some diseases, mostly cancers, associated SNPs are preferentially located in TAD borders. Cancers are relatively more frequent among these diseases, and this relative predominance is enhanced when considering only intergenic SNPs. Network analysis demonstrates that these results are not due to a small number of SNPs common to several diseases.
The fact that the associated SNP is not necessarily the causal variant and may be only a marker related to the causal variation through linkage disequilibrium (LD) does not affect significantly our conclusion: The correlated variations would still be located in the neighborhood of the corresponding TAD border, whose size (20 kb) is often larger than or comparable to the range of strong LD [44, 45]. Moreover, LD has been partly eliminated by the selection of SNP arrays used in GWAS and manual curation in the GWAS catalog. We checked these features by computing, for each disease, the number of pairs of SNPs located at a distance closer than a given threshold, of values ranging from 10 kb to 1 Mb (Additional file 1: Table S2). The resulting low numbers confirm that LD can only be a minor contribution in the interpretation of our observations. In any case, a wider genetic variation (more extended along the genome than a single point mutation, as would follow from linkage disequilibrium) or a correlated genetic variation across a TAD border  would even make more plausible an involvement of an architectural change including the TAD border in the risk of developing the disease.
While TAD organization in cancer cells is globally largely intact, many studies have shown that chromatin architecture can be disrupted in cancer by changes in TAD boundaries due to the vast genetic alterations, including copy number variation, mutations, translocations that accompany cancer development and progression (reviewed, e.g., in ). Such disruptions lead to aberrant gene expression within the affected TADs. However, very little is known about how genetic variations associated with cancer susceptibility are favoring cancer development in healthy individuals (i.e., before cancer development) and how they impact 3D genome organization. Using capture Hi-C approaches, it was shown that GWAS SNP variants associated with cancer in breast , prostate  and in colorectal tissues  affect long-range chromatin interactions. These pioneer studies suggested that such genetic variants drive altered expression of certain oncogenes and tumor suppression genes, but their impact was so far restricted to chromatin loop organization within TADs. Our results suggest a different mechanism of genetic risk for cancers and non-cancer diseases. Specifically, cancers might be promoted by a joint mis-regulation of oncogenes through a weakening of TAD borders, while non-cancer diseases would rather be favored by local mis-regulations of specific genes (and their cascading consequences).
The weaker relationship between genetic risk loci and TAD borders observed in hESC is consistent with the experimental evidence in mammals of a progressive maturation of the internal structure of TADs, with the establishment of additional enhancer-promoter interactions and further sub-TAD structures during cell differentiation [38, 40]. In other cell lines investigated, TAD-border enrichment in intergenic daSNPs discriminates cancers and non-cancer diseases and suggests an essential difference about the role of genome organization in TADs as regards their genetic risk. These results will now have to be confronted with recent advances in understanding the full complexity of 3D genome organization, its cell-type dependence and its influence on gene regulation [49, 50].
Our investigation demonstrates a link between genetic risk and 3D genome partitioning into topologically insulated domains. A genetic variation located in TAD borders may weaken the insulation of adjacent TADs, which prevents spurious interactions between genes and enhancers located in adjacent TADs. The larger frequency of cancers among these diseases supports the importance of TAD border weakening in a subset of cancers and emphasizes the different type of gene mis-regulation involved in cancer etiology. A cancer generally involves the malfunction of numerous genes, which is more readily achieved by the extended deregulations induced by weakening of a single TAD border rather than affecting each gene individually. TAD disruption induced by somatic mutations has been observed in cancers , and our results suggest that an at-risk variant SNP (and its correlated variations) may act as a head start.
Our results offer the proof-of-concept of a novel criterion for filtering SNPs according to their 3D genomic location, and identifying especially relevant associations, i.e., SNP prioritization. Our study opens a new research avenue in the personalized diagnosis of genetic risk, based on the interplay between 3D genome organization and the location of at-risk SNPs. Dissecting the functional correlates of the preferential location of risk loci in TAD borders now challenges experimental studies. Various experiments, including genome editing to monitor the allelic form of specific loci and chromosome conformation capture techniques, could bring a mechanistic support to this novel statistical evidence of a link between 3D genome organization and the risk of developing certain complex diseases. Our analysis there provides a guideline for experimental studies, in suggesting candidate loci where the mechanisms underlying the genetic risk may involve the effect of the genetic variation on the 3D genome structure.
Disease-associated SNPs (daSNPs)
We used the version v1.0.2-associations_e94_r2018-09-30 of the GWAS catalog: www.ebi.ac.uk/gwas/ . We extracted all SNP entries associated with a disease EFO term (Experimental Factor Ontology), overall 449 EFO terms, distinguishing 71 cancers and 378 non-cancer diseases. Specifically, diseases were found by selecting all traits which fall into the disease subtree (EFO_0000408) from the EFO ontology, and subsequently, cancers are separated from non-cancer diseases by using the cancer subtree (EFO_0000311).
A SNP can be associated with a disease multiple times in the GWAS catalog when it was found in distinct studies; we ignored this multiplicity by dropping duplicates of its identifier snpId.
We classified the resulting 21,183 daSNPs into intergenic (40%), intronic (55%) or exonic (5%) type according to its parent category in The Sequence Ontology database, (http://www.sequenceontology.org), version 2015-11-24 .We then identified for each disease the subset of its associated intergenic SNPs (on average 47 daSNPs and 18 intergenic daSNPs for both cancer and non-cancer diseases).
We used the Cooler Hi-C database  at ftp://cooler.csail.mit.edu/coolers, which provides published Hi-C data files in the.cool format, at 10 kb-resolution (bin size). Throughout our study, genomic coordinates refer to the hg19 genome version adopted in this database. We present in the main text results obtained with high-resolution data from E. Lieberman-Aiden’s laboratory  for the five native and non-cancerous cell lines available, namely GM12878 (human lymphoblastoid cell line, data obtained with MboI or DpnII restriction enzyme), IMR90 (fetal lung fibroblasts of Caucasian origin), HMEC (human mammary epithelial cells), NHEK (normal human epidermal keratinocytes) and HUVEC (human umbilical vein endothelial cells). We considered only normal cell types (and not cancer cell types) as we are interested in the genetic risk present at birth in all cells and wanted to exclude possible confounding features appearing during cancer development itself. We also investigated datasets from B. Ren’s laboratory [19, 36,37,38], obtained in pioneering experiments using a lower sequencing depth and an enzyme HindIII producing larger restriction fragments (see Additional file 1: Fig. S8), for several cell types: GM12878 in , IMR90 in [19, 36] embryonic stem cells (H1 hESC) in [19, 38], and cell lines derived from H1 hESC in , namely mesendoderm (H1_ME), neural progenitors (H1_NP) trophoblast-like cells (H1_TB) and mesenchymal cells (H1_MS). Overall, 15 datasets were examined in our study.
We determined TAD coordinates using the TopDom algorithm , applied after a transformation of.cool files into count matrices using https://github.com/open2c/cooler. Its principle is to count the number of contacts in a window sliding along the genome and to locate TAD ends at the minima of this count (see Fig. 1, blue diamonds). Genomic regions having established only very few contacts in the experiment, labeled ‘gap’ by TopDom, were filtered out. The choice of using this algorithm is supported by comparative studies [33,34,35]. Moreover, TopDom is based on quantifying the topological insulation between adjacent TADs, which is the feature that matters for gene (mis)regulation. In particular, recently evidenced long-range associations between TADs and their higher-order organization  will not be considered here.
The TAD caller thus involves a tunable parameter k, measuring the half-size (in bins, of length equal to the chosen resolution) of the sliding window. This parameter offers a way to investigate the well-known variability in TAD determination [33,34,35], as depicted in Additional file 1: Fig. S2. The general trend is that larger numbers of TADs are observed for lower values of k, at which substructures are also extracted while only large TADs are extracted by the algorithm at large values of k. To overcome this technical variability, we scanned all values of the window size k from k = 3 to k = 20 and adopted two strategies: either to aggregate our observations over these values of k (Fig. 2), or to use a more stringent majority rule in further analyses (Figs. 3, 4). Both strategies reduce small-number effects and smooth out TAD variability, overall yielding robust results despite the lack of robustness of the TAD landscape (Additional file 1: Fig. S2 and Additional file 1: Fig. S3).
A discrepancy between TADs determined with TopDom and the visual impression given by the contact map could appear locally (see, e.g., Fig. 1), coming from the following difference: TopDom is based on the insulation of TADs, i.e., the presence of low-density (yellow) zones between the triangles delineating the TADs (see Fig. 1), whereas the alternative understanding of a TAD as a region with an increased density of internal contacts would rather focus on dark red triangles emerging from the background in the Hi-C map.
We defined TAD borders as regions of 20 kb located inside the TAD at the limits of this TAD. Their size has been chosen smaller than the median size (23 kb) of a human gene .
This definition agrees with a topological characterization of TAD borders as regions across which the contact frequency displays a marked decrease  and is consistent with the use of TopDom for calling TADs. It differs from the notion of TAD boundary, considered, e.g., in [19, 24] which is the—not always existing—linker region between two successive TADs along the genome (not belonging to any TAD), whereas a TAD will always have two borders. TAD borders cover from 8% up to 14% of the genome when TopDom parameter k varies, the smallest fraction being observed for k = 20 (see Additional file 1: File S2, Additional file 1: Table S3 and, for the variation according to the Hi-C dataset, Additional file 1: Table S4). Additionally, to get an idea of the order of magnitude of the numbers involved, the average number of border SNPs for various subsets of diseases (cancers vs non-cancer diseases, displaying or not TAD-border enrichment), and the broad range in which this number varies due to some outlier extreme values, is given in Additional file 1: File S2, together with some figures about SNPs subcategories (exonic, intronic or intergenic).
Preferential location of daSNPs in TAD borders (TAD-border enrichment)
For each disease (EFO term), we have tested whether the associated SNPs are located in TAD borders more often than observed by chance, where chance (i.e., the null model in statistical terms) corresponds to the same number of pointwise loci drawn at random in the entire genome. In a second analysis, chance corresponds to the same number of SNPs drawn at random in the entire set of disease-associated SNPs listed in the GWAS catalog. The statistical significance of a preferential location of daSNPs in TAD borders is then assessed by computing a p-value for the disease according to a hypergeometric test.
In more detail:
Testing a preferential location in TAD borders of the SNPs associated to a given disease involves the hypergeometric distribution H (q | N, n, Q) describing the probability of getting q border SNPs when drawing Q elements (as many as the number of SNPs associated to the considered disease) at random, without replacement, in the null model. In the genome-based null model, N is the total number of base pairs in the genome and n the part located in TAD borders. In the SNP-based null model, close to that considered, e.g., in , N is the total number of disease-associated SNPs listed in the GWAS catalog (each counted once, and not including the SNPs associated with non-pathological traits so that the random model is the closest possible to the data) and n the part located in TAD borders. The computation for testing TAD-border enrichment is performed at the SNP level, i.e., at the base-pair level: Two SNPs located in the same bin, or in the same border, will be counted as two units. These occurrences are very rare events (see Additional file 1: Table S2). Results presented in the main text were obtained with the more conservative genome-based null model. A comparison with those obtained with the SNP-based null model is presented in Additional file 1: Fig. S4.
The p-value for the considered disease, assessing the over-representation of its associated SNPs in TAD borders, is computed as the cumulative distribution function (i.e., the fraction of values larger than or equal to q) of this hypergeometric distribution. Given the symmetry property H (q | N, n, Q) = H (q | N, Q, n) of the hypergeometric distribution, it is equivalent to state that (i) the SNPs associated with the disease are located in a TAD border more often than expected by chance, or (ii) TAD borders contain a SNP associated with this disease more often than expected by chance. We term such a situation TAD-border enrichment.
After computing a raw p-value for each disease, Benjamini–Hochberg procedure (multipletests function with method fdr_bh from the statsmodels package in Python, www.statsmodels.org/dev/index.html#citation) is applied to obtain p-values adjusted for multiple testing, so that the false discovery rate is controlled at level 5% when the adjusted p-value is lower than 0.05 . Given the overwhelming number of non-cancer diseases (378) compared to cancers (71) and their different etiology, we investigated separately these two groups of diseases. These two groups are well-defined on biological criteria independently of our enrichment testing, so that correction for multiple testing has been applied separately in each group. Nevertheless, we checked that our main result (TAD-border enrichment in daSNPs for certain diseases and its relatively higher frequency for cancers) remains qualitatively observed when we used a global multiple-testing correction, considering jointly cancers and non-cancer diseases, or even no correction (Additional file 1: Fig. S5 and Additional file 1: Fig. S6).
Histograms of corrected p-values (Fig. 2) are plotted and normalized separately for cancers and non-cancer diseases. The counts have been first aggregated over the six considered datasets from  and the values of the window parameter k of the TAD caller. In order to get a better display of the core features of the plots, the range of p-values has been truncated at log10(1/p) = 4. A few EFOs, with corrected p-values smaller than 0.0001, are thus lying outside the displayed plot. It would be possible to choose a larger bin size or even to draw a smooth histogram, but this would rather dilute the information.
Comparison of TAD-border enrichment for cancers and non-cancer diseases
Due to the small number of cancers (71) compared to non-cancer diseases (378), we compared the fraction of cancers and the fraction of non-cancer diseases displaying a significant TAD-border enrichment, considering either all their associated SNPs or only a sub-category (exonic, intronic and intergenic). Only diseases displaying a significant enrichment for a majority (more than 50%) of values of the window parameter k have been counted. The significance of a difference between the disease fractions has been assessed using Fisher’s exact test. The comparison has been done for each of the six considered datasets (for 5 cell types) from , and also after aggregating the disease counts over these datasets.
The different steps described above have been gathered in an easy-to-execute pipeline, using Snakemake (https://snakemake.github.io, ) unifying the analysis of different datasets. Its rule graph is presented on Additional file 1: Fig. S1. Its code, written in Python, is freely available at https://github.com/kpj/GeneticRiskAndTADs. An order of magnitude of the typical numbers of diseases and SNPs of different categories involved in our analysis is provided in Additional file 1: File S2, Additional file 1: Table S3, Additional file 1: Table S4.
SNP-based diseasome network and its analysis
We introduced a network representation where nodes are diseases and an edge is drawn between two diseases when they share an associated SNP. Starting from a bipartite network relating diseases and their associated SNPs, this representation is the projection on disease nodes. It is the analog for SNPs of the network relating diseases and their associated genes, known as the diseasome, and its projected version . A filter has been applied on shared SNPs: in the network labeled ‘border SNPs,’ an edge is drawn when the diseases share a SNP lying in a TAD border for a majority of values of the window parameter k (underlying Hi-C data from , IMR90 cell type). The network labeled ‘non-border SNPs’ involves the complementary set of SNPs. Additionally, the two networks have been re-drawn considering only intergenic SNPs. Non-cancer disease and cancer nodes (and among them, those displaying TAD-border enrichment) were underlined with different colors. Note that, each cancer or each non-cancer disease is associated with an ensemble of border SNPs, of non-border SNPs, of intergenic border SNPs and of intergenic non-border SNPs, and could be present in more than one of the four networks. In Fig. 5, the four networks have been visualized using NetworkX Python package. For each network, only diseases (nodes) having an associated SNP of the prescribed type are drawn.
For a quantitative comparison of the four networks regarding their clustering and assortativity properties, we computed an indicator for any subset of nodes, e.g., a group of nodes with the same color. This indicator, called network coherence, is defined as the z-score of the number of edges within the subset of nodes, compared to a thousand randomly drawn groups of nodes of the same size [42, 43]. Network coherence thus measures whether the induced subgraph is more densely connected (i.e., contains more links) than expected at random in the original network. As a z-score, it provides an absolute quantification, independent of the overall size of the group, which makes cross-comparisons possible. Choosing a threshold larger than 1 on the number of shared SNPs required to draw an edge does not change qualitatively the results but reduces the number of diseases involved, which brings statistics to a limit. All the results presented in the text were obtained with a threshold equal to 1.
Availability of data and materials
The datasets supporting the conclusions of this article are available in the Cooler repository: https://github.com/open2c/cooler (datasets gathered in.cool format  from published studies [19, 30, 36,37,38]. The pipeline specifically devised for our study is available at https://github.com/kpj/GeneticRiskAndTADs.
Genome-wide association studies
Human embryonic stem cell
Genome-wide chromosome conformation capture
Human fetal lung fibroblasts
Topologically associating domain
Hardy J, Singleton A. Genome wide association studies and human disease. New Engl J Med. 2009;360:1759–68.
Visscher PM, Wray NR, Zhang Q, Sklar P, McCarthy MI, Brown MA, et al. 10 years of GWAS discovery: biology, function, and translation. Am J Hum Genet. 2017;101:5–22.
Erichsen HC, Chanock SJ. SNPs in cancer research and treatment. Brit J Cancer. 2004;90:747–51.
McKay JD, Hung R, Han Y, Zong X, Carreras-Torres R, Christiani D, et al. Large-scale association analysis identifies new lung cancer susceptibility loci and heterogeneity in genetic susceptibility across histological subtypes. Nat Genet. 2017;49:1126–32.
Maurano MT, Humbert R, Thurman RE, Haugen E, Wang H, Reynolds AP, et al. Systematic localization of common disease-associated variation in regulatory DNA. Science. 2012;337:1190–5.
Ji X, Dadon DB, Powell BE, Fan ZP, Borges-Rivera D, Shachar S, et al. 3D chromosome regulatory landscape of human pluripotent cells. Cell Stem Cell. 2016;18:262–75.
Nasser J, Bergman DT, Fulco CP, Guckelberger P, Doughty BR, Patwardhan TA, et al. Genome-wide enhancer maps link risk variants to disease genes. Nature. 2021;593:238–43.
Downes DJ, Schwessinger R, Hill SJ, Nussbaum L, Scott C, Gosden ME, et al. An integrated platform to systematically identify causal variants and genes for polygenic human traits. BioRxiv. 2019;27:813618. https://doi.org/10.1101/813618.
Cano-Gamez E, Trynka G. From GWAS to function: using functional genomics to identify the mechanisms underlying complex diseases. Front Genet. 2020;11:424.
Lappalainen T, Sammeth M, Friedländer MR, Ac‘t Hoe P, Monlong J, Rivas MA, et al. Transcriptome and genome sequencing uncover functional variation in humans. Nature. 2013;501:506–11.
Pombo A, Dillon N. Three-dimensional genome architecture: players and mechanisms. Nat Rev Mol Cell Biol. 2015;16:245–57.
Roy SS, Mukherjee AK, Chowdhury S. Insights about genome function from spatial organization of the genome. Hum Genomics. 2018;12:1–9.
Gorkin D, Qiu Y, Hu M, Fleter-Brant K, Liu T, Schmitt A, et al. Common DNA sequence variation influences 3-dimensional conformation of the human genome. Genome Biol. 2019;20:255.
Malod-Dognin N, Pancaldi V, Valencia A, Pržulj N. Chromatin network markers of leukemia. Bioinformatics. 2020;36:i455–63.
Kikuchi M, Hara N, Hasegawa M, Miyashita A, Kuwano R, Ikeuchi T, et al. Enhancer variants associated with Alzheimer’s disease affect gene expression via chromatin looping. BMC Med Genomics. 2019;12:128.
Verlaan DJ, Berlivet S, Hunninghake GM, Madore AM, Larivière M, Moussette S. Allele-specific chromatin remodeling in the ZPBP2/GSDMB/ORMDL3 locus associated with the risk of asthma and autoimmune disease. Am J Hum Genet. 2009;85:377–93.
Berlivet S, Moussette S, Ouimet M, Verlaan DJ, Koda V, Al Tuwaijri A, et al. Interaction between genetic and epigenetic variation defines gene expression patterns at the asthma-associated locus 17q12-q21 in lymphoblastoid cell lines. Hum Genet. 2012;131:1161–71.
Stadhouders R, Aktuna S, Thongjuea S, Aghajanireafah A, Pourzafad F, van Ljcken W, et al. HBS1L-MYB intergenic variants modulate fetal hemoglobin via long-range MYB enhancers. J Clin Invest. 2014;124:1699–710.
Dixon JR, Selvaraj S, Yue F, Kim A, Li Y, Shen Y, et al. Topological domains in mammalian genomes identified by analysis of chromatin interactions. Nature. 2012;485:376–80.
Willemin A, Lopez-Delisle L, Bolt CC, Gadolini ML, Duboule D, Rodriguez-Carballo E. Induction of a chromatin boundary in vivo upon insertion of a TAD border. PLOS Genet. 2021;17:e1009691.
Lupiáñez DG, Kraft K, Heinrich V, Krawitz P, Brancati F, Klopocki E, et al. Disruptions of topological chromatin domains cause pathogenic rewiring of gene-enhancer interactions. Cell. 2015;161:1012–25.
Ea V, Sexton T, Gostan T, Herviou L, Baudement M-O, Zhang Y, Berlivet S, Le Lay-Taha M-N, Cathala G, Lesne A, Victor J-M, Fan Y, Cavalli G, Forné T. Distinct polymer physics principles govern chromatin dynamics in mouse and Drosophila topological domains. BMC Genomics. 2015;6:607.
Krijger PHL, de Laat W. Regulation of disease-associated gene expression in the 3D genome. Nat Rev Mol Cell Biol. 2016;17:771–82.
Sun JH, Zhou L, Emerson DJ, Phyo SA, Titus KR, Gong W, et al. Disease-associated short tandem repeats co-localize with chromatin domain boundaries. Cell. 2018;175:224–38.
Lonfat N, Montavon T, Darbellay F, Gitto S, Duboule D. Convergent evolution of complex regulatory landscapes and pleiotropy at Hox loci. Science. 2014;346:1004–6.
Mozziconacci J, Merle M, Lesne A. The 3D genome shapes the regulatory code of developmental genes. J Mol Biol. 2019;432:712–23.
Valton A-L, Dekker J. TAD disruption as oncogenic driver. Curr Op Genet Dev. 2016;36:34–40.
Achinger-Kawecka J, Clark SJ. Disruption of the 3D cancer genome blueprint. Epigenomics. 2017;9:47–55.
Jablonski KP, Fretter C, Carron L, Forné T, Hütt MT, Lesne A. Genome supranucleosomal organization and genetic susceptibility to diseases. AIP Conf Proc. 2017;1882:020027.
Rao SS, Huntley MH, Durand NC, Stamenova EK, Bochkov ID, Robinson JT. A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping. Cell. 2014;159:1665–80.
Shin H, Shi Y, Dai C, Tjong H, Gong K, Alber F, et al. TopDom: an efficient and deterministic method for identifying topological domains in genomes. Nucleic Acids Res. 2016;44:1–13.
McArthur E, Capra JA. Topologically associating domain boundaries that are stable across diverse cell types are evolutionarily constrained and enriched for heritability. Am J Hum Genet. 2021;108:269–83.
Dali R, Blanchette M. A critical assessment of topological associating domain prediction tools. Nucleic Acid Res. 2017;45:2994–3005.
Forcato M, Nicoletti C, Pal K, Livi CM, Ferrari F, Bicciato S. Comparison of computational methods for Hi-C data analysis. Nat Methods. 2017;14:679.
Zufferey M, Tavernari D, Oricchio E, Ciriello G. Comparison of computational methods for the identification of topologically associating domains. Genome Biol. 2018;19:217.
Jin F, Li Y, Dixon JR, Selvaraj S, Ye Z, Lee AY, et al. A high-resolution map of the three-dimensional chromatin interactome in human cells. Nature. 2013;503:290.
Selvaraj S, Dixon JR, Bansal V, Ren B. Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing. Nat Biotechnol. 2013;31:1111–8.
Dixon JR, Jung I, Selvaraj S, Shen Y, Antosiewicz-Bourget JE, et al. Chromatin architecture reorganization during stem-cell differentiation. Nature. 2015;518:331–6.
Rowley MJ, Corces VG. The three-dimensional genome: principles and roles of long-distance interactions. Curr Op Cell Biol. 2016;40:8–14.
Oudelaar AM, Beagrie RA, Gosden M, de Ornellas S, Georgiades E, Kerry J, et al. Dynamics of the 4D genome during in vivo lineage specification and differentiation. Nat Commun. 2020;11:1–12.
Goh KI, Cusick ME, Valle D, Childs B, Vidal M, Barabási L. The human disease network. Proc Natl Acad Sci USA. 2017;104:8685–90.
Sonnenschein N, Dzib JFG, Lesne A, Eilebrecht S, et al. A network perspective on metabolic inconsistency. BMC Syst Biol. 2012;6:41.
Knecht C, Fretter C, Rosenstiel P, Krawczak M, Hütt MT. Distinct metabolic network states manifest in the gene expression profiles of pediatric inflammatory bowel disease patients and controls. Sci Rep. 2016;6:1–11.
Gabriel SB, Schaffner SF, Nguyen H, Moore JM, Roy J, Blumenstiel B, et al. The structure of haplotype blocks in the human genome. Science. 2002;296:2225–9.
Whalen S, Pollard KS. Most chromatin interactions are not in linkage disequilibrium. Genome Res. 2019;29:334–43.
Dryden NH, Broome LR, Dudbridge F, et al. Unbiased analysis of potential targets of breast cancer susceptibility loci by capture Hi-C. Genome Res. 2014;24:1854–68.
Du M, Tillmans L, Gao J, et al. Chromatin interactions and candidate genes at ten prostate cancer risk loci. Sci Rep. 2016;6:23202.
Jäger R, Migliorini G, Henrion M, et al. Capture Hi-C identifies the chromatin interactome of colorectal cancer risk loci. Nat Commun. 2015;6:6178.
Szabo Q, Bantignies F, Cavalli G. Principles of genome folding into topologically associating domains. Sci Adv. 2019;5:eaaw1668. https://doi.org/10.1126/sciadv.aaw1668.
Ibrahim DM, Mundlos S. The role of 3D chromatin domains in gene regulation: a multi-facetted view on genome organization. Curr Opin Genet Dev. 2020;61:1–8.
Buniello A, MacArthur JAL, Cerezo M, Harris LW, Hayhurst J, Malangone C, et al. The NHGRI-EBI GWAS catalog of published genome-wide association studies, targeted arrays and summary statistics. Nucleic Acids Res. 2019;47:D1005–12.
Eilbeck K, Lewis S, Mungall C, Yandell M, Stein L, Durbin R, et al. The sequence-ontology: a tool for the unification of genome annotations. Genome Biol. 2005;6:R44.
Abdennur N, Mirny L. Cooler: scalable storage for Hi-C data and other genomically labeled arrays. Bioinformatics. 2019;36:311–3.
Paulsen J, Ali TML, Nekrasov M, Delbarre E, Baudement MO, Kurscheid S, et al. Long-range interactions between topologically associating domains shape the four-dimensional genome during differentiation. Nat Genet. 2019;51:835–43.
Scherer S. Guide to the human genome. Cold Spring Harbor Laboratory Press; 2010.
Ea V, Baudement MO, Lesne A, Forné T. Contribution of topological domains and loop formation to 3D chromatin organization. Genes. 2015;6:734–50.
Kundaje A, Meuleman W, Ernst J, Bilenky M, Yen A, Heravi-Moussavi A, et al. Integrative analysis of 111 reference human epigenomes. Nature. 2015;518:317–30.
Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc B Met. 1995;57:289–300.
Mölder F, Jablonski KP, Letcher B, Hall MB, Tomkins-Tinch CH, Sochat V, et al. Sustainable data analysis with Snakemake. F1000Research. 2021;10:33.
The authors thank Nastasija Mijovic, Cosette Rebouissou, Valentin Ruault, Marina Villaverde, and the members of the CNRS GDR 3536 ‘ADN’ (https://adn-g.fr) for insightful discussions.
This work has been supported by the “Mission for Interdisciplinarity and Transverse Initiatives” (https://miti.cnrs.fr) of the French National Center for Scientific Research (CNRS), program InFIniTI 2017 & 2018, project 3D-SNPs, Grant 232647 (to AL), by the “Agence Nationale de la Recherche” (https://anr.fr), project CHRODYT, Grant ANR-16-CE15-0018-04 (to TF) and by INCa-Cancéropôle GSO, (http://www.canceropole-gso.org) grant 2018-E08 (to AL). L. Carron acknowledges the Ministry of Higher Education, Research and Innovation (https://www.enseignementsup-recherche.gouv.fr) for having funded his PhD at LPTMC and the “Agence Nationale de la Recherche” (https://anr.fr), grant ANR 18-CE13-004, for funding his current postdoctoral position at LCQB. M.T. Hütt thanks LPTMC (Paris) for hospitality and the Physics Institute of CNRS (https://inp.cnrs.fr/en) for funding his stays, during which part of this work has been performed. MTH also acknowledges financial support from the German Ministry for Education and Research (https://www.bmbf.de/en), sysINFLAME project within the e:med program, grant 01ZX1606D). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information including: Fig. S1: Rule graph of the integrated pipeline. File S1: Lists of cancers displaying TAD border enrichment in their associated SNPs. Fig. S2: Variation of TADs and TAD borders at varying TopDom parameter k. Fig. S3: Variation of TAD and TAD borders across data sources. Fig. S4: Comparison of two null models for assessing TAD border enrichment in daSNPs. Fig. S5: Multiple testing correction in assessing TAD border enrichment in daSNPs. Fig. S6: Multiple testing correction in assessing TAD border enrichment in intergenic daSNPs. Fig. S7: TAD border enrichment across data sources. Table S1: Values of network coherence for subgraphs in SNP-based diseasome networks. Table S2: Analysis of pairwise distance between daSNPs. Fig. S8: Restriction fragment size distributions. File S2: Typical numbers of diseases and SNPs involved in the analysis. Table S3: Genome fraction located in TAD borders. Table S4: Genome fraction and number of SNPs in TAD borders for different datasets.
About this article
Cite this article
Jablonski, K.P., Carron, L., Mozziconacci, J. et al. Contribution of 3D genome topological domains to genetic risk of cancers: a genome-wide computational study. Hum Genomics 16, 2 (2022). https://doi.org/10.1186/s40246-022-00375-2